SCAN_Guide

We collected and manually curated extensive nervous system sequencing data and developed a comprehensive, easily accessible, and user-friendly database (SCAN), thus providing a convenient and efficient data mining framework. Uniquely, the SCAN database combines both scRNA-seq and ST datasets for the nervous systems of multiple species, while also integrating genomic, and epigenetic data. Currently, SCAN includes scRNA-seq datasets of the nervous system of 67 species, as well as multiomics data of 900 species. The database is divided into four functional sections. The "Atlas Section" presents an expansive atlas of neural systems, detailing species-specific information, single-cell data, and spatially resolved transcriptomic, genomic, and transcription factor binding data. The "Biology Section" explores evolutionary aspects, developmental trajectories, stemness characteristics, metabolism, genetic regulatory networks, and cellular microenvironments of neural systems. The "Annotation Section" integrates tools, such as Celltypist and SingleR , for cell type annotation. The "Disease Section" provides a single-cell atlas of over 100 neural diseases, as well as disease fingerprint analysis and putative biomarker identification at the single-cell level. Overall, SCAN is a comprehensive database providing transformative information to facilitate advancements in neuroscience research.